4 6 diamidino 2 phenylindole dapi nucleic acid stain (Thermo Fisher)

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Thermo Fisher 4 6 diamidino 2 phenylindole dapi nucleic acid stain
4 6 Diamidino 2 Phenylindole Dapi Nucleic Acid Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nucleic+acid+stain+dapi/4'%2C6'-Diamidino-2-phenylindole+dihydrochloride%2C+98%25/pmc13107922-309-10-15
Average 99 stars, based on 1 article reviews

4 6 diamidino 2 phenylindole dapi nucleic acid stain - by Bioz Stars, 2026-09

99/100 stars

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Staining:

Article Title: On the role of the plasmodial cytoskeleton in facilitating intelligent behavior in slime mold Physarum polycephalum
Article Snippet: .. Samples were mounted in antifade reagent containing the fluorescent nucleic acid stain DAPI (4′,6-Diamidino-2-phenylindole dilacetate) (Molecular Probes, USA) and imaged with a Perkin-Elmer UltraView FRET-H spinning disk confocal microscope. .. The primary antibodies used were KMX-1 (anti-myxamoebal β-tubulin; Millipore, UK) at a concentration of 4 μg/ml and ACTN05(C4) (anti-actin; Abcam, UK) at 2 μg/ml.

Article Title: Neuronal and Astrocytic Extracellular Vesicle Biomarkers in Blood Reflect Brain Pathology in Mouse Models of Alzheimer’s Disease
Article Snippet: Then, sections were washed three times in TBS-T and incubated for 1 h at room temperature with a 1:200 dilution of Alexa Fluor TM 488 goat anti-mouse secondary antibody (cat. no. A11001, Thermo Fisher Scientific) in blocking solution. .. Excess secondary antibody was removed by washing slides three times in TBS-T followed by the addition of mounting media supplemented with the nucleic acid stain DAPI (cat. no. P36962, Thermo Fisher Scientific) prior to visualization of sections and image acquisition using the Zeiss LSM 710 confocal laser scanning microscope system (Carl Zeiss Inc., Jena, Germany). ..

Article Title: Drug treatment alters performance in a neural microphysiological system of information processing.
Article Snippet: .. Cells were again washed three times and the nucleic acid stain DAPI (ThermoFisher, Australia) was briefly added for 5min prior to visualization to indicate the presence of cells. .. Cultures were visualized using aNikonA1Plus Ti ZDrive confocal microscope (Nikon, Japan), and at least Fig. 7 | Network summary statistics using all of the recorded channels under control and different drug treatments.

Article Title: ISG15 as a prognostic biomarker in solitary fibrous tumour
Article Snippet: The following secondary fluorescent antibodies were used at 1:1000 for 1 h at RT: Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 and Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 (Invitrogen). .. Nucleic acid stain DAPI (Invitrogen) was subsequently added for 15 min. After careful washing, coverslips were mounted onto microscope slides with ProLongTM Gold mounting media (ThermoFisher). .. Fluorescence microscope Olympus BX-61 was used for image acquiring.

Article Title: Myricetin possesses the potency against SARS-CoV-2 infection through blocking viral-entry facilitators and suppressing inflammation in rats and mice.
Article Snippet: RBDWT or mutant proteins were mixed with 0, 100, 200, or 300 μM myricetin and incubated for 40 min, and Fc-tagged (Invitrogen, Carlsbad, CA, USA) or His-Tagged antibody (Cell Signaling Technology, Boston, MA, USA) added and incubated for another 120 min. .. The nucleic acid stain DAPI (Thermo Fisher Scientific, Waltham, MA, USA) was used to label the cell nuclei 5 min before images were acquired with the Carl Zeiss LSM780 system (Zeiss, Oberkochen, Germany) as previously described (Chu et al., 2020). .. Pseudo typed lentiviral particle assay HEK293 cells that express human ACE2 via transfection with pLV [Exp]–mCherry/NeoEF1A>hACE2[nm_02184.3] (Vector Builder, Guangzhou, China) were called HEK293-hACE2-mCherry cells.

Article Title: Myricetin possesses the potency against SARS-CoV-2 infection through blocking viral-entry facilitators and suppressing inflammation in rats and mice
Article Snippet: RBD WT or mutant proteins were mixed with 0, 100, 200, or 300 μM myricetin and incubated for 40 min, and Fc-tagged (Invitrogen, Carlsbad, CA, USA) or His-Tagged antibody (Cell Signaling Technology, Boston, MA, USA) added and incubated for another 120 min. .. The nucleic acid stain DAPI (Thermo Fisher Scientific, Waltham, MA, USA) was used to label the cell nuclei 5 min before images were acquired with the Carl Zeiss LSM780 system (Zeiss, Oberkochen, Germany) as previously described ( ). .. HEK293 cells that express human ACE2 via transfection with pLV[Exp]–mCherry/NeoEF1A>hACE2[nm_02184.3] (Vector Builder, Guangzhou, China) were called HEK293-hACE2-mCherry cells.

Article Title: Drug treatment alters performance in a neural microphysiological system of information processing
Article Snippet: .. Cells were again washed three times and the nucleic acid stain DAPI (ThermoFisher, Australia) was briefly added for 5 min prior to visualization to indicate the presence of cells. .. Cultures were visualized using a Nikon A1Plus Ti ZDrive confocal microscope (Nikon, Japan), and at least three images (2048 x 2048 pixels with 161 z-steps of 0.7 μm) were recorded per well.

Microscopy:

Laser-Scanning Microscopy:

other:

Article Title: Comparative analysis of SARS-CoV-2 Omicron BA.2.12.1 and BA.5.2 variants.
Article Snippet: School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, P.R.. China State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, School of Clinical Medicine, Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, P.R.. China Department of Infectious Disease and Microbiology, The University of Hong Kong‐Shenzhen Hospital, Shenzhen, Guangdong Province, P.R.

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